MATLAB Answers

0

Invalid use of operator.

Asked by Raviteja Kurva on 18 Jan 2019
Latest activity Commented on by Walter Roberson
on 18 Jan 2019
% 1 step: create slice and save resulting img
% 2 step: run registation
%% create slice and substract dark image
% readim
myfile1 = imread('cam14-blank181112_2018-11-12-115852-0000.jpg');
% dark image subtraction
%dark1 = readtimeseries('dark\camera1\camera1_2015-03-28-204209-0000.tif');
%dark1 = dark1{1};
%myfile1 = myfile1 - squeeze(mean(dark1{1},[],3));
%myfile1 = myfile1 - squeeze(mean(dark1));
dark1 = imread('cam14-blankNoLight181112_2018-11-12-120006-0000.jpg');
myfile1 = myfile1 - dark1;
imwrite(myfile1,'slice_cam14-blank181112_2018-11-12-115852-0000','jpg',0,[])
myfile2 = imread('cam14-blank181112_2018-11-12-115852-0001.jpg');
% dark image subtraction
%dark2 = readtimeseries('dark\camera2\camera2_2015-03-28-204250-0000.tif');
%dark2 = dark2{1};
%myfile2 = myfile2 - squeeze(mean(dark2{1},[],[3 4]));
%myfile2 = myfile2 - squeeze(mean(dark2{1}, [], 3));
dark2 = imread('cam14-blankNoLight181112_2018-11-12-120006-0001.jpg');
myfile2 = myfile2 - dark2;
imwrite(myfile2,'slice_cam14-blank181112_2018-11-12-115852-0001','jpg',0,[])
%%
[separated,transform]=PreprocessPicoSIM('slice_cam14-blank181112_2018-11-12-115852-0000.jpg', 'slice_cam14-blank181112_2018-11-12-115852-0001.jpg');
%%
% save aligned images
stack=squeeze(separated);
Filebase='stack1\\img%03d.tif';
for num=0:size(stack,3)-1;
Filename=sprintf(Filebase,num);
tiffwrite(Filename,squeeze(stack(:,:,num)),'no');
end
% save transformation parameters
% save transform.mat transform
Hello I am very new to programming. please help me out with this. thanks in Advance

  0 Comments

Sign in to comment.

1 Answer

Stephan
Answer by Stephan
on 18 Jan 2019
Edited by Stephan
on 18 Jan 2019

Hi,
try:
% 1 step: create slice and save resulting img
% 2 step: run registation
%% create slice and substract dark image
% readim
myfile1 = imread ('cam14-blank181112_2018-11-12-115852-0000.jpg');
% dark image subtraction
% dark1 = readtimeseries ('dark \ camera1 \ camera1_2015-03-28-204209-0000.tif');
% dark1 = dark1 {1};
% myfile1 = myfile1 - squeeze (mean (dark1 {1}, [], 3));
% myfile1 = myfile1 - squeeze (mean (dark1));
dark1 = imread ('cam14-blankNoLight181112_2018-11-12-120006-0000.jpg');
myfile1 = myfile1 - dark1;
imwrite (myfile1, 'slice_cam14-blank181112_2018-11-12-115852-0000', 'jpg')
myfile2 = imread ('cam14-blank181112_2018-11-12-115852-0001.jpg');
% dark image subtraction
% dark2 = readtimeseries ('dark \ camera2 \ camera2_2015-03-28-204250-0000.tif');
% dark2 = dark2 {1};
% myfile2 = myfile2 - squeeze (mean (dark2 {1}, [], [3 4]));
% myfile2 = myfile2 - squeeze (mean (dark2 {1}, [], 3));
dark2 = imread ('cam14-blankNoLight181112_2018-11-12-120006-0001.jpg');
myfile2 = myfile2 - dark2;
imwrite (myfile2, 'slice_cam14-blank181112_2018-11-12-115852-0001', 'jpg')
%%
[separated, transform] = PreprocessPicoSIM ('slice_cam14-blank181112_2018-11-12-115852-0000.jpg', 'slice_cam14-blank181112_2018-11-12-115852-0001.jpg');
%%
% save aligned images
stack = squeeze (separated);
Filebase = 'stack1 \\ img% 03d.tif';
for num = 0: size (stack, 3) -1
Filename = sprintf (Filebase, num);
tiffwrite (Filename, squeeze (stack (:,:, num)), 'no');
end
% save transformation parameters
% save transform.mat transform
Best regards
Stephan

  5 Comments

Walter Roberson
on 18 Jan 2019
what are you expecting the 0, [] to signify ?
Stephan
on 18 Jan 2019
following the documentation for imwrite there are no option that can be specified the way you do. i edited my answer this way.
More errors?
i do not find a Matlab function named tiffwrite for 2018b. is it your own function or from another source? maybe you are looking for write.
Walter Roberson
on 18 Jan 2019
tiffwrite appears in some third party contribution including in some textbooks that supply their own libraries .

Sign in to comment.