I need to count cell nucleus in these images how can I do that

Can I get a code in image processing field to count the cells’ nucleus from these photos

Answer 1

[Vinayak Agrawal]

Hi Asia,

Yes, here is an example code in MATLAB for counting the cells' nucleus from a JPEG photo:

% Load the image

img = imread('cells.jpg');

% Convert the image to grayscale

grayimg = rgb2gray(img);

% Apply a median filter to remove noise

medimg = medfilt2(grayimg);

% Enhance contrast using histogram equalization

equalizedimg = histeq(medimg);

% Segment the image using adaptive thresholding

thresimg = adaptthresh(equalizedimg, 0.3);

binaryimg = imbinarize(equalizedimg, thresimg);

% Remove small objects from the binary image

binaryimg = bwareaopen(binaryimg, 10);

% Find the connected components in the binary image

cc = bwconncomp(binaryimg);

% Count the number of cells' nucleus

nucleusCount = cc.NumObjects;

% Display the results

figure;

subplot(2,2,1); imshow(img); title('Original Image');

subplot(2,2,2); imshow(medimg); title('Grayscale Image with Median Filtering');

subplot(2,2,3); imshow(equalizedimg); title('Contrast Enhanced Image');

subplot(2,2,4); imshow(binaryimg); title(['Detected Nuclei: ', num2str(nucleusCount)]);

You may need to adjust the parameters of the image processing functions and methods used in this code to obtain the desired results for your input image. Also, note that this code assumes a certain level of expertise in image processing and MATLAB programming

Hope it helps

[Image Analyst]

The histogram equalization step is not needed. It is almost never needed in any situation.