I am trying to use background subtraction on a set of images in a folder

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I am new to the image processing side of MATLAB. The images I am trying to subtract the background from are a set of frames from a video. I have successfully subtracted the background from one using a different photo I constructed in the paint app (the area of interest is highlighted in white, leaving just that area after the subtraction (this is quite important). Since the camera is stationary, I need to use the same singular photo to carry out background subtraction on all 95 frames. I managed to somewhat do this using the code below, however the images were put into a cell, therefore I could not save the images, see them individually, or watch them as a video. Is this possible to do automatically, subtracting the background from 95 different images using the same one image to subtract from, since repeating this process in paint 95 times would take a long time? Or is there a way to remove the images from the cell where I can save them, see them individually and watch them together as a video? Thanks
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Andrew Palmer
Andrew Palmer on 9 Aug 2022
Sorry, the code that gave me the subtracted images in a cell is below since I forgot to attach.
filePattern=fullfile('C:\', 'folder1', 'folder2','*.jpg');
for k = 1:95
fullFileName=fullfile(images1(k).folder, baseFileName);
fprintf(1, 'now reading %s/n', fullFileName);
for k = 2:95

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Accepted Answer

Image Analyst
Image Analyst on 9 Aug 2022
Try this:
folder = fullfile('C:\folder1\folder2');
filePattern = fullfile(folder, '*.jpg');
fileList = dir(filePattern);
numberOfFiles = numel(fileList);
subtractionFileName = 'subtract.jpg'; % or fullfile(folder, 'subtract.jpg') if it's in the same folder.
if ~isfile(subtractionFileName)
errorMessage = sprintf('Subtraction image not found:\n%s', subtractionFileName);
backgroundImage = imread(subtractionFileName);
for k = 1:numberOfFiles
baseFileName = fileList(k).name;
if contains(baseFileName, 'subtract', 'IgnoreCase',true)
fullFileName = fullfile(fileList(k).folder, baseFileName);
fprintf(1, 'Now reading %s/n', fullFileName);
thisImage = imread(fullFileName);
% Do the subtraction. Use subtractionImage{k} if you need to save them all for some reason
% and you have enough memory to save them all.
subtractionImage = thisImage - backgroundImage;
% Display the image.
imshow(subtractionImage, []);
caption = sprintf('Image #%d of %d : %s', k, numberOfFiles, baseFileName);
title(caption, 'Interpreter','none');
drawnow; % force it to update the screen immediately.
Image Analyst
Image Analyst on 10 Aug 2022
You can save the images one at a time with imwrite() or else you can save the whole cell array into a .mat file with save() and then recall it with load().

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