stage_file_maker

stage_file_maker is a MATLAB GUI app that to generate the stage file (.STG) used by MetaMorph.

stage_file_maker is a MATLAB GUI app that to generate the stage file (.STG) used by MetaMorph.

This app is developed for 2D stage screening on microscope controlled by MetaMorph.

It has pre-saved recommended step sizes for from the single well dish to 96-well plate under 10X, 20X and 40X objective. One can also revise the screening step size by changing the X and Y values on the interface.

The app generates a rectangle position matrix for each well based on the row (R) and column (C) numbers given to the interface. “Offset” is the offset of the Perfect Focus System (PFS).

The “Generate” button generates the position matrix. The upper-left corner of the whole screening is set to (0, 0, 0) as the relative position. Correspondingly, one needs to manually define the upper-left corner of the screening area on the sample as the origin (0, 0, 0) in MetaMorph.

“Save as” button saves the positions into a “.STG” file ready to be loaded to the “Stage” tab of the “Multi Dimensional Acquisition” app in MetaMorph.

By default, the Z coordinates of all the positions are set to zero. One can enter the actual Z coordinates of the four corners of the screening area and click the “Update” button. In that way, the Z coordinate of each position is calculated accordingly and updated in the internal memory.