How to get the first derivative of pixels along of line?

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#Here's my code
#it works for one frame when I did 'plot(diff(test))' but I need a way to implement it to the whole images
[x ,y]= ginput(2);
dif = zeros(size(M,3),1);
for i = 1:size(M,3)
I = M(:,:,i);
n = ceil(norm([diff(x), diff(y)]));
test = improfile(I,linspace(x(1), x(2),n), linspace(y(1), y(2),n));
dif(i) = diff(test);
BA on 12 Aug 2022
@RikI'd like to do some attempts and then reframe the question.

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Answers (1)

KSSV on 12 Aug 2022
REad about gradient

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