Attempting to open a script to analyze a PTU file

Hello everyone, I am currently trying to analyze a PTU file called "c3_c5_DNA_1.ptu" using a script provided to me called "Read_PTU_V1". I have no experience programming in MatLab and limited experience in other languages. I have tried modifying the parts of the script I believe I need to edit to make it work, but I've been unsuccessful up to the moment. The following lines correspond to the parts of the script I believe need to be modified. The only changes I made are adding the path of the files to where I believed the script needed them. I have attached copies of those two sections for reference.

Original Script:

function [output, txtout]=(filepath) % Read PicoQuant Unified TTTR Files

My Change:

function [output, txtout]=('C:\Users\jgodinez6\Desktop\Read_PTU_V1') % Read PicoQuant Unified TTTR Files

Another section (original):

fid=fopen(filepath);

My change:

fid=fopen('C:\Users\jgodinez6\Desktop\c3_c5_DNA_1.ptu');

This is the error I get when I try to run it:

>> Read_PTU_V1
Error: File: Read_PTU_V1.m Line: 1 Column: 39
Unexpected MATLAB expression.

I will really appreciate your support. Thank you!

Answer 1

[Geoff Hayes]

Jose - the problem is with the signature of this function

function [output, txtout]=(filepath) 

While you are providing the input and output parameter names, you are not supplyinbg a function name. See Declare function name, inputs, and outputs for details on how to do this. In your case, you would do something like

function [output, txtout] = myFunction(filepath) 

and save this to a file myFunction.m. This is an example only...you can name it to be whatever you want but the function name must match the file name.

You would then call this function from the command line (or another script or function) as

>> [output, txtout] = myFunction('C:\Users\jgodinez6\Desktop\Read_PTU_V1') 

where you pass in the path and name of the function that you want to process. Since you are passing in this string, then this is the value of the filepath input parameter, and so the line to open the file can still remain as

fid=fopen(filepath);

[Jose Godinez]

I think your suggestion solved part of the issue. However, it still indicates there is an error on the path. This is what is showing me:

Error: File: Read_PTU_V1.m Line: 1 Column: 39
Invalid expression. Check for missing multiplication operator, missing or unbalanced delimiters, or other
syntax error. To construct matrices, use brackets instead of parentheses.

Thi is line 1:

function [output, txtout]=Read_PTU_V1('C:\Users\josec\OneDrive\Documents\MATLAB')

[Geoff Hayes]

Jose - your function signature (line 1) is incorrect

function [output, txtout]=Read_PTU_V1('C:\Users\josec\OneDrive\Documents\MATLAB')

In the above you, need to define the input parameter as a variable and not as a string. You pass the string when you call the function. So your line 1 must become

function [output, txtout]=Read_PTU_V1(filepath)

where filepath is the input parameter for this function which will then be evaluated in the code. You set this input parameter with a value when you call the function from the command line (or from another script or function) as

>> [output, txtout]=Read_PTU_V1('C:\Users\josec\OneDrive\Documents\MATLAB')

Please see the attached simple example.

[Jose Godinez]

I think this worked. Thank you!

[abdul kalam]

Hi

I am using the matlab function Read_PTU_V1 (filepath) written by Omri Bar-Elli to read a .ptu file recorded with Picoharp260 in T3 mode. Please see the link attched for the reference (https://www.mathworks.com/matlabcentral/fileexchange/61789-read_ptu_v1-filepath).

While using the function, I am getting an error as "Illegal RecordType!"

Can someone help me in reading the file complaetely?

Thank you very much in advance.